NutriStem™ hPSC XF Culture Medium for Human iPS and ES Cells

NutriStem hPSC XF Culture Medium is a fully-defined, xeno-free, low growth factor concentration, feeder-free culture medium for human embryonic stem (ES) and induced pluripotent stem (iPS) cells. Cells can be cultured for at least 20 passages while retaining pluripotency marker expression, robust proliferation with a normal karyotype, and the ability to differentiate into cells of all three germ layers in vitro and in vivo.

NutriStem hPSC XF Medium (formerly called NutriStem XF/FF Culture Medium) is a fully defined xeno-free, low growth factor human embryonic stem (ES) and induced pluripotent stem (iPS) cell feeder-free culture medium that enables maintenance and expansion of pluripotent stem cells. NutriStem Medium can be used to culture pluripotent stem cells for at least 20 passages while retaining pluripotency marker expression, robust proliferation with a normal karyotype, and the ability to differentiate into cells of all three germ layers in vitro and in vivo. NutriStem Medium offers the ability to culture cells in a completely xeno-free medium without the need for high levels of basic FGF and other stimulatory growth factors and cytokines. In addition, the superior cell attachment and proliferation observed with NutriStem Medium aid high-throughput screening applications.

• Xeno-free, feeder-free conditions contain no animal components
• Easy, one-step transition from feeder-dependent culture, no adaptation required
• Maintains pluripotency, normal morphology, karyotype, and differentiation potential of human ES and iPS cells over long term culture
• Robust attachment and high cloning efficiency from single cells
• Amenable to a weekend-free culture