DPBS, (10x) w/o: Ca and Mg

DPBS, (10x) w/o: Ca and Mg

Equivalent/Alternative to: Biochrom Cat.-no. L 1835 more…

Cat-no : P04-53500

Size: 500 ml

Store at: Room Temperature

Sterile : Yes

HS-Code: 38229000

Availability:  Green

File : DPBS, (10x) w/o: Ca and Mg | P04-53500 (pan-biotech.de)

About

Dulbecco’s Phosphate-Buffered Saline, commonly known as DPBS, is an indispensable reagent in cellular research. This saline solution is balanced with vital ions and is free of calcium and magnesium. Its uses and applications are manifold, making DPBS a staple in many cell culture laboratories worldwide.

DPBS serves primarily as a buffer and maintains the delicate pH balance necessary for biological reactions. Cells are sensitive entities, and any fluctuation in their environment can lead to unfavorable outcomes. This is where DPBS steps in, providing a consistent environment that mimics a cell’s natural surroundings, ensuring that experiments proceed without external pH-induced variations.

Another fundamental use of DPBS is in the washing of cells. During various stages of cell culture and experimentation, cells need to be separated from their medium, either for the replacement of the old medium or to prepare them for downstream applications. DPBS provides a gentle washing solution that removes residues without harming the cells. The absence of calcium and magnesium in DPBS ensures that cells do not clump together during this process, facilitating easier subsequent manipulations.

DPBS also plays a significant role in cell dissociation. In processes where enzymatic methods like trypsin are employed to dissociate cells, DPBS acts as a rinsing agent prior to the addition of the enzyme. This ensures that any residual medium or impurities are removed, optimizing the efficiency of the dissociation process.

Moreover, DPBS finds its use in resuspending cells. After centrifugation, cells are often resuspended in DPBS, especially if they are being prepared for applications like flow cytometry. Its isotonic nature ensures that cells remain viable during resuspension.

Lastly, DPBS acts as a carrier or diluent for many reagents, ensuring that they are in a biologically-friendly and physiologically relevant solution before being introduced to cells.

In conclusion, the multifaceted utility of DPBS in cellular research cannot be understated. From maintaining pH to washing and resuspending cells, DPBS is a silent workhorse that ensures cellular experiments proceed with accuracy and reproducibility.