Cat N-terminal pro-brain natriuretic peptide, NT-proBNP ELISA Kit

Cat N-terminal pro-brain natriuretic peptide, NT-proBNP ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store at 2 to 8 degree C for 6 months.

Manufactured in an ISO 9001:2015 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Small volumes of NT-proBNP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1602649 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the N-terminal pro-brain natriuretic peptide, NT-proBNP, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NT-proBNP. The ELISA analytical biochemical technique of the MBS1602649 kit is based on NT-proBNP antibody-NT-proBNP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NT-proBNP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NT-proBNP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: This sandwich kit is for the accurate quantitative detection of Cat N-terminal pro-brain natriuretic peptide (also known as NT-proBNP) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Cat NT-proBNP antibody. NT-proBNP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Cat NT-proBNP Antibody is added and binds to NT-proBNP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NT-proBNP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Cat NT-proBNP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.