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การเพาะเลี้ยงเนื้อเยื่อ
ผงทริปซิน (1 : 250) มาจากหมู
Cytokine
BINKIT for NK cells expansion from PBMCs
สารเคมี
Demecolcine solution 10 µg/ml
Dimethylsulfoxide (DMSO) for cell culture
Penicillin-Streptomycin-Amphotericin B Mix, 10,000 U/ml Penicillin, 10 mg/ml Streptomycin, 25µg/ml Amphotericin B in 0,85% Saline
Dispase II (Neutral Protease, Grade II) From Bacillus Polymyxa in PBS, w/o: Ca, Mg, 2.4 U/ml
Paneticin G 418, Powder
Dimethylsulfoxide (DMSO) for cell culture, Endotoxin tested
Bovine Serum Albumin (BSA), US Origin, Fatty acid free, lyophilized powder
EDTA 1:5000 (1X), 0.2 g/L in PBS, w/o Ca and Mg
Trypan blue 0.4 % solution
PHA-L Phytohemagglutinin, 1.2 mg Protein/Flask, lyophilized
เอนไซม์
Trypsin 0.25 %/EDTA 0.02 %, in PBS w/o: Ca and Mg, w: Phenol red
Antibiotics
Penicillin-Streptomycin/Amphotericin B Mix, 10,000 U/ml Penicillin, 10 mg/ml Streptomycin, 25µg/ml Amphotericin B in 0,85% Saline
Cryopreservation
Cryopan I, Serum-free Freezing Medium, w/o: Human and Animal Components
Trypsin 0.05 %/EDTA 0,1 % in PBS, w/o: Ca and Mg
Freezing medium, Cryopreservation-Medium, w: DMSO, w: FBS
Erythrocyte Lysis Buffer 1x
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