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สื่อของเหลว
DMEM, w: 4.5 g/L Glucose, w: stable Glutamine, w: Sodium pyruvate, w: 3.7 g/L NaHCO3, Very Low Endotoxin
สารเคมี
PHA-L Phytohemagglutinin, 1.2 mg Protein/Flask, lyophilized
Sera
FBS Premium, Australia origin, fetal bovine serum, 0.2 µm sterile filtered
RPMI 1640, w: L-Glutamine, w/o: NaHCO3, Powder
เอนไซม์
Trypsin 0.25 %/EDTA 0.02 %, in PBS w/o: Ca and Mg, w: Phenol red
บัฟเฟอร์
HBSS, w/o: Ca and Mg, w/o: Phenol red, w: 0.35 g/L NaHCO3
MEM Eagle w: EBSS, w: L-Glutamine, w: 1.5 g/L NaHCO3
สื่อมวลชน
Alpha MEM Eagle, w: L-Glutamine, w: Ribonucleosides, w: Deoxyribonucleosides, w: 2.2g/L NaHCO3
Antibiotics
Penicillin-Streptomycin/Amphotericin B Mix, 10,000 U/ml Penicillin, 10 mg/ml Streptomycin, 25µg/ml Amphotericin B in 0,85% Saline
Fetal Bovine Serum, Australia, gamma irradiated
Chicken Serum, sterile filtered
RPMI 1640, w/o: L-Glutamine, w/o: NaHCO3, Powder
Human Serum, sterile filtered
Cryopreservation
Cryopan I, Serum-free Freezing Medium, w/o: Human and Animal Components
DMEM, w: 4.5 g/L Glucose, w: L-Glutamine, w: 25 mM HEPES, w/o: Sodium pyruvate, w: 3.7 g/L NaHCO3
Trypsin 0.05 %/EDTA 0,1 % in PBS, w/o: Ca and Mg
DMEM, w: 4.5 g/L Glucose, w: L-Glutamine, w: Sodium pyruvate, w/o: Phenol red, w: 3.7 g/L NaHCO3
Freezing medium, Cryopreservation-Medium, w: DMSO, w: FBS
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