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FBS Premium
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Sera

FBS Premium, Australia origin, fetal bovine serum, 0.2 µm sterile filtered

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สื่อของเหลว

RPMI 1640, w: L-Glutamine, w/o: NaHCO3, Powder

100mL bottle of trypsin/edta solution. The red color is due to phenol.
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เอนไซม์

Trypsin 0.25 %/EDTA 0.02 %, in PBS w/o: Ca and Mg, w: Phenol red

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บัฟเฟอร์

HBSS, w/o: Ca and Mg, w/o: Phenol red, w: 0.35 g/L NaHCO3

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สื่อของเหลว

MEM Eagle w: EBSS, w: L-Glutamine, w: 1.5 g/L NaHCO3

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สื่อมวลชน

Alpha MEM Eagle, w: L-Glutamine, w: Ribonucleosides, w: Deoxyribonucleosides, w: 2.2g/L NaHCO3

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Antibiotics

Penicillin-Streptomycin/Amphotericin B Mix, 10,000 U/ml Penicillin, 10 mg/ml Streptomycin, 25µg/ml Amphotericin B in 0,85% Saline

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Sera

Fetal Bovine Serum, Australia, gamma irradiated

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Sera

Chicken Serum, sterile filtered

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สื่อของเหลว

RPMI 1640, w/o: L-Glutamine, w/o: NaHCO3, Powder

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Sera

Human Serum, sterile filtered

Clear plastic bottle with white cap containing yellow solution.
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Cryopreservation

Cryopan I, Serum-free Freezing Medium, w/o: Human and Animal Components

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สื่อของเหลว

DMEM, w: 4.5 g/L Glucose, w: L-Glutamine, w: 25 mM HEPES, w/o: Sodium pyruvate, w: 3.7 g/L NaHCO3

Small bottle containing clear solution. The solution is trypsin and EDTA mixuture.
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เอนไซม์

Trypsin 0.05 %/EDTA 0,1 % in PBS, w/o: Ca and Mg

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สื่อของเหลว

DMEM, w: 4.5 g/L Glucose, w: L-Glutamine, w: Sodium pyruvate, w/o: Phenol red, w: 3.7 g/L NaHCO3

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Cryopreservation

Freezing medium, Cryopreservation-Medium, w: DMSO, w: FBS

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สารเคมี

Erythrocyte Lysis Buffer 1x

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การเพาะเลี้ยงเนื้อเยื่อ

MEM Eagle w: EBSS, w/o: L-Glutamine, w: 2.2 g/L NaHCO3

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