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Human PBMCs stained for flow cytometry with antibody specific for myeloperoxidase
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Antibodies

Anti-Hu Myeloperoxidase FITC

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Reagents

Demecolcine solution 10 µg/ml

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Reagents

Dimethylsulfoxide (DMSO) for cell culture

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Sera

Human Serum off-the-clot, Type AB, male, sterile filtered

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Reagents

Penicillin-Streptomycin-Amphotericin B Mix, 10,000 U/ml Penicillin, 10 mg/ml Streptomycin, 25µg/ml Amphotericin B in 0,85% Saline

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Tissue Culture

DMEM/F12 (1:1), w: L-Glutamine, w: 15 mM HEPES, w: 1.2 g/L NaHCO3

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Reagents

Dispase II (Neutral Protease, Grade II) From Bacillus Polymyxa in PBS, w/o: Ca, Mg, 2.4 U/ml

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Sera

Rabbit Serum, sterile filtered

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Reagents

Paneticin G 418, Powder

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Tissue Culture

Ham´s F12 Medium, w: L-Glutamine, w: 1.176 g/L NaHCO3

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Reagents

Dimethylsulfoxide (DMSO) for cell culture, Endotoxin tested

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Tissue Culture

MEM Eagle w: EBSS, w: stab. Glutamine, w: 0.85 g/L NaHCO3

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Tissue Culture

RPMI 1640, w: L-Glutamine, w/o: Phenol red, w: 2.0 g/L NaHCO3

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Sera

FBS Premium, South America origin, activated charcoal absorbed, 0.2 µm sterile filtered

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Tissue Culture

IMDM, w/o: L-Glutamine, w: 25 mM HEPES, w: 3.024 g/L NaHCO3

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Sera

NBC, Australia origin, newborn calf serum, 0.2 µm sterile filtered

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Buffer

HEPES Buffer, Powder

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Sera

Human Serum, Type AB, male, sterile filtered

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