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Overlapping flow cytometry histogram of CD314 APC positive cells compared to a negative population
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Antibodies

Anti-Hu CD314 APC

Dot plot of human PBMCs stained wiht CD235a and a secondary antibody
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Antibodies

Anti-Hu CD235a APC-Cy7

Flow cytometric dual plot of side scatter versus CD34 fitc staining
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Antibodies

Anti-Hu CD34 FITC

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Antibodies

Anti-Hu CD45 Purified

Quad plot of CD133 and CD34 flow cytometric staining
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Antibodies

Anti-Hu CD133 PE

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Antibodies

Anti-Hu CD146 PE

Human PBMCs stained for flow cytometry with antibody specific for myeloperoxidase
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Antibodies

Anti-Hu Myeloperoxidase FITC

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Reagents

Demecolcine solution 10 µg/ml

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Reagents

Dimethylsulfoxide (DMSO) for cell culture

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Sera

Human Serum off-the-clot, Type AB, male, sterile filtered

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Reagents

Penicillin-Streptomycin-Amphotericin B Mix, 10,000 U/ml Penicillin, 10 mg/ml Streptomycin, 25µg/ml Amphotericin B in 0,85% Saline

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Tissue Culture

DMEM/F12 (1:1), w: L-Glutamine, w: 15 mM HEPES, w: 1.2 g/L NaHCO3

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Reagents

Dispase II (Neutral Protease, Grade II) From Bacillus Polymyxa in PBS, w/o: Ca, Mg, 2.4 U/ml

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Sera

Rabbit Serum, sterile filtered

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Reagents

Paneticin G 418, Powder

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Tissue Culture

Ham´s F12 Medium, w: L-Glutamine, w: 1.176 g/L NaHCO3

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Reagents

Dimethylsulfoxide (DMSO) for cell culture, Endotoxin tested

Pan Tm
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Tissue Culture

MEM Eagle w: EBSS, w: stab. Glutamine, w: 0.85 g/L NaHCO3

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