Total RNA Purification Kit

Description: Total RNA Purification Kit is designed for rapid, high purity and high yield isolation of total RNA from small amounts of various samples including blood, animal and plant tissue, bacteria and viruses.
The spin column based method allows complete removal of inhibitors such as divalent cations and proteins. Due to elimination of phenol, handling of the kit is safe and no harmful waste is produced. The purified total RNA can be used in a number of downstream applications. The kit allows the purification of up to 100 μg RNA per preparation.

Content:
Lysis Buffer (before use, add 2-Mercaptoethanol as indicated on the bottle) – stable for 1 month at room temperature.
Activation Buffer
Blood Washing Buffer (before use, add 96-99 % Ethanol as indicated on the bottle)
First Washing Buffer (before use, add 96-99 % Ethanol as indicated on the bottle)
Second Washing Buffer (before use, add 96-99 % Ethanol as indicated on the bottle)
Elution Buffer
Spin Columns
2 ml Collection Tubes

To be provided by you:
2-Mercaptoethanol (2-ME)
Optional: Chloroform
96-99 % Ethanol
2-Propanol (Isopropanol)
1.5 ml microtubes

Preparation procedure:
Before start, add the following components (not included in the kit) as indicated on the respective bottle:

• 2-Mercaptoethanol to the Lysis Buffer (10 μl 2-Mercaptoethanol per 1 ml Lysis Buffer)
• 96-99 % Ethanol to Blood Washing Buffer, First Washing Buffer and Second Washing Buffer

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