IL-1 alpha (Interleukin-1α), Human

Sequence:
MSAPFSFLSNVKYNFMRIIKYEFILNDALNQSIIRANDQYLTAAALHNLDEAVKFDMGAYKSSKDDAKITVILRISKTQLYVTAQDEDQPV
LLKEMPEIPKTITGSETNLLFFWETHGTKNYFTSVAHPNLFIATKQDYWVCLAGGPPSITDFQILENQA with polyhistidine tag at the C-terminusSource:
Escherichia coliEndotoxin Test:
<0.1 EU per 1 μg of the protein by the LAL method.Activity:
Measure by its ability to induce D10.G4.1 cells proliferation. The ED50 for this effect is <10 pg/mL. The specific activity of recombinant human IL-1 alpha is approximately >1 x108 IU/mg.Purity:
>98% as determined by SDS-PAGE.

Form:
Lyophilized

Storage Buffer:
Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.

Reconstitution:
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

Stability & Storage:
This product is stable after storage at:
• -20°C for 12 months in lyophilized state from date of receipt.
• -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.