Human Dermal Fibroblast – Adult (HDF-Ad)
฿40,590.00
General information
Description | Human Dermal Fibroblasts, Adult (HDF-Ad), are primary cells isolated from the dermis layer of adult human skin. These cells play a crucial role in skin physiology, being responsible for the production of extracellular matrix components, including collagen and elastin, which are essential for maintaining skin structure and function. HDF-Ad cells are frequently utilized in research related to wound healing, aging, and tissue engineering, given their significant role in skin repair and regeneration processes. Additionally, they serve as an important model for studying fibroblast behavior in various dermatological conditions and diseases.
HDF-Ad cells are highly responsive to external stimuli, making them a valuable tool for investigating the cellular responses to different environmental factors such as UV radiation, oxidative stress, and various pharmaceutical compounds. Their ability to proliferate and produce essential proteins under controlled conditions also makes them suitable for studies in drug development, particularly in the context of dermal toxicity and efficacy testing. These cells retain many of the physiological characteristics of their tissue of origin, providing a relevant model for in vitro studies aimed at understanding skin biology at the molecular and cellular levels. |
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Organism | Human |
Tissue | Dermis
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Handling
Culture Medium | MEM, w/o ribonucleosides, w/o deoxyribonucleosides (We do not supply this product; please consider other suppliers. Please let us know if you need further assistance) |
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Medium supplements | Supplement the medium with 10% FBS, 2 ng/mL hr-bFGF, 2 mM stable L-glutamine |
Passaging solution | Trypsin-EDTA |
Subculturing | Remove the culture medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add trypsin-EDTA 0.25% solution, 1-2ml per T25, 2.5ml per T75 cell culture flask, the cell sheet must be covered completely, and incubate at 37 degree Celsius for 10 min. Stop the trypsin activity using FBS-containing cell culture medium. Dispense into new flasks which contain fresh cell culture medium. |
Seeding density | 1 to 3*10^3 cells/cm² |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Vendor | CLS – Cell Lines Service, Germany |
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