Anti-Hu CD56 Alexa Fluor® 700

• Isotype
  Mouse IgG2a
• Specificity
  The mouse monoclonal antibody LT56 recognizes an extracellular epitope of CD56 (NCAM), a transmembrane glycoprotein expressed ubiquitously in the nervous system and found also on T cells and NK cells.
• Workshop
  HLDA X
• Application
  FC (QC tested)
• Application details
  Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Reactivity
  Human
• Immunogen
  Cell line KG1a
• Preparation
  Purified antibody is conjugated with Alexa Fluor^® 700 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Formulation
  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Storage and handling
  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Description:

CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of immunoglobulin family serving as adhesive molecule which is ubiquitously expressed in nervous system, usually as 120 kDa, 140 kDa or 180 kDa isoform, and it is also found on T cells and NK cells. Polysialic modification results in reduction of CD56-mediated cell adhesion and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumours and ovarian granulosa cell tumours.

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