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Small clear plastic bottle with clear solution of sodium bicarbonate chemical.
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Buffer

Sodium bicarbonate 7,5 %

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Reagents

Trypan blue 0.4 % solution

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Tissue Culture

Ham’s F10 Medium, w: L-Glutamine, w: 25 mM HEPES, w/o: NaHCO3, Powder

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Liquid Media

DMEM, w: 1.0 g/L Glucose, w: L-Glutamine, w: Sodium pyruvate, w/o: Phenol red, w: 3.7 g/L NaHCO3

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Liquid Media

DMEM, w: 4.5 g/L Glucose, w: stable Glutamine, w: Sodium pyruvate, w: 3.7 g/L NaHCO3, Very Low Endotoxin

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Reagents

PHA-L Phytohemagglutinin, 1.2 mg Protein/Flask, lyophilized

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Liquid Media

RPMI 1640, w: L-Glutamine, w/o: NaHCO3, Powder

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Buffer

HBSS, w/o: Ca and Mg, w/o: Phenol red, w: 0.35 g/L NaHCO3

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Liquid Media

MEM Eagle w: EBSS, w: L-Glutamine, w: 1.5 g/L NaHCO3

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Media

Alpha MEM Eagle, w: L-Glutamine, w: Ribonucleosides, w: Deoxyribonucleosides, w: 2.2g/L NaHCO3

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Liquid Media

RPMI 1640, w/o: L-Glutamine, w/o: NaHCO3, Powder

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Liquid Media

DMEM, w: 4.5 g/L Glucose, w: L-Glutamine, w: 25 mM HEPES, w/o: Sodium pyruvate, w: 3.7 g/L NaHCO3

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Liquid Media

DMEM, w: 4.5 g/L Glucose, w: L-Glutamine, w: Sodium pyruvate, w/o: Phenol red, w: 3.7 g/L NaHCO3

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Tissue Culture

MEM Eagle w: EBSS, w/o: L-Glutamine, w: 2.2 g/L NaHCO3

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Reagents

Dimethylsulfoxide (DMSO)

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Liquid Media

DMEM/F12 (1:1), w/o: L-Glutamine, w: 1.2 g/L NaHCO3

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Buffer

DPBS, w/o: Ca and Mg, Powder

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Liquid Media

DMEM, w: 1.0 g/L Glucose, w/o: L-Glutamine, w: Sodium pyruvate, w: 3.7 g/L NaHCO3

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