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Liquid Media
DMEM/F12 (1:1), w: L-Glutamine, w/o: Phenol red, w: 1.2 g/L NaHCO3
Enzyme
Trypsin powder (1 : 250) porcine origin
Media
DMEM:Ham’s F12 with 5% FBS
Freeze Medium CM-1- 50 ml
Tissue Culture
Centrifuge Tubes 50ML
Centrifuge Tubes 15ML
Reagents
Demecolcine solution 10 µg/ml
Dimethylsulfoxide (DMSO) for cell culture
DMEM/F12 (1:1), w: L-Glutamine, w: 15 mM HEPES, w: 1.2 g/L NaHCO3
Ham´s F12 Medium, w: L-Glutamine, w: 1.176 g/L NaHCO3
Dimethylsulfoxide (DMSO) for cell culture, Endotoxin tested
MEM Eagle w: EBSS, w: stab. Glutamine, w: 0.85 g/L NaHCO3
RPMI 1640, w: L-Glutamine, w/o: Phenol red, w: 2.0 g/L NaHCO3
IMDM, w/o: L-Glutamine, w: 25 mM HEPES, w: 3.024 g/L NaHCO3
Buffer
HBSS, w: Ca and Mg, w/o: NaHCO3, Powder
McCoy’s 5A Medium (modified), w: L-Glutamine, w: 2.2 g/L NaHCO3
M199 w: EBSS, w: L-Glutamine, w: 2.2 g/L NaHCO3
Ham’s F-10 w/o: phenol red, w: 1.2 g/l NaHCO3, w: 10 mM HEPES
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