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4 quadrant dot plot of CD3 vs CD56 showing NK cell expansion
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Cytokine

BINKIT for NK cells expansion from PBMCs

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Reagents

Demecolcine solution 10 µg/ml

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Reagents

Dimethylsulfoxide (DMSO) for cell culture

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Reagents

Penicillin-Streptomycin-Amphotericin B Mix, 10,000 U/ml Penicillin, 10 mg/ml Streptomycin, 25µg/ml Amphotericin B in 0,85% Saline

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Reagents

Dispase II (Neutral Protease, Grade II) From Bacillus Polymyxa in PBS, w/o: Ca, Mg, 2.4 U/ml

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Reagents

Paneticin G 418, Powder

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Reagents

Dimethylsulfoxide (DMSO) for cell culture, Endotoxin tested

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Bovine Serum Albumin (BSA), US Origin, Fatty acid free, lyophilized powder

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Reagents

EDTA 1:5000 (1X), 0.2 g/L in PBS, w/o Ca and Mg

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Reagents

Trypan blue 0.4 % solution

Pan Tm
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Reagents

PHA-L Phytohemagglutinin, 1.2 mg Protein/Flask, lyophilized

100mL bottle of trypsin/edta solution. The red color is due to phenol.
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Enzyme

Trypsin 0.25 %/EDTA 0.02 %, in PBS w/o: Ca and Mg, w: Phenol red

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Antibiotics

Penicillin-Streptomycin/Amphotericin B Mix, 10,000 U/ml Penicillin, 10 mg/ml Streptomycin, 25µg/ml Amphotericin B in 0,85% Saline

Clear plastic bottle with white cap containing yellow solution.
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Cryopreservation

Cryopan I, Serum-free Freezing Medium, w/o: Human and Animal Components

Small bottle containing clear solution. The solution is trypsin and EDTA mixuture.
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Enzyme

Trypsin 0.05 %/EDTA 0,1 % in PBS, w/o: Ca and Mg

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Cryopreservation

Freezing medium, Cryopreservation-Medium, w: DMSO, w: FBS

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Reagents

Erythrocyte Lysis Buffer 1x

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Reagents

Dimethylsulfoxide (DMSO)

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